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Centre for Structural and Functional Genomics


7141 Rue Sherbrooke Ouest

Montréal, Quebec H4B 1R6

The Kwan Lab


Leclerc LMY, Soffer G, Kwan DH, Shih SCC. A fucosyltransferase inhibition assay using image-analysis and digital microfluidics.

Biomicrofluidics. 2019 May 10;13(3):034106. doi: 10.1063/1.5088517.

Zhang X, Chen F, Petrella A, Chacón-Huete F, Covone J, Tsai TW, Yu CC, Forgione P, Kwan DH. A High-Throughput Glycosyltransferase Inhibition Assay for Identifying Molecules Targeting Fucosylation in Cancer Cell-Surface Modification. ACS Chem Biol. 2019 Apr 19;14(4):715-724. doi

Tegl G, Hanson J, Chen HM, Kwan DH, Santana AG, Withers SG. Facile formation of β-thioGlcNAc Linkages to Thiol-Containing Sugars, Peptides, and Proteins using a Mutant GH20 HexosaminidaseAngew Chem Int Ed Engl. 2019 Feb 4;58(6):1632-1637. doi


David H. Kwan. Structure-Guided Directed Evolution of Glycosidases: A Case Study in Engineering a Blood Group Antigen-Cleaving Enzyme. Methods Enzymol. 2017;597:25-53. doi.


David H. Kwan, Yi Jin, Jianbing Jiang, Hong-Ming Chen, Miriam P. Kötzler, Herman S. Overkleeft, Gideon J. Davies, and Stephen G. Withers. Chemoenzymatic synthesis of 6-phospho-cyclophellitol as a novel probe of 6-phospho-β-glucosidases. Febs Letters. 590(4):461-8. doi


Gesa Volkers, Liam J. Worrall, David H. Kwan, Ching-Ching Yu, Lars Baumann, Emilie Lameignere, Gragory A. Wasney, Nicollas E. Scott, Warren Wakarchuk, Leonard J. Foster, Stephen G. Withers, and Natalie C.J. Strynadka. Structure of human ST8SiaIII sialyltransferase provides insight into cell-surface polysialylation. Nature Structural & Molecular Biology. 22(8):627-35. doi

David H. Kwan, Sabrina Ernst, Miriam P. Kötzler, and Stephen G. Withers. Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes. Glycobiology. 25(8):806-11. doi

David H. Kwan, Iren Constantinescu, Rafi Chapanian, Melanie A. Higgins, Miriam P. Kötzler, Eric Samain, Alisdair B. Boraston, Jayachandran N. Kizhakkedathu, and Stephen G. Withers. Toward Efficient Enzymes for the Generation of Universal Blood through Structure-Guided Directed Evolution. Journal of the American Chemical Society. 137(17):5695-705. doi


Rafi Chapanian, David H. Kwan, Iren Constantinescu, Fathima A. Shaikh, Nicholas A.A. Rossi, Stephen G. Withers, and Jayachandran N. Kizhakkedathu. Enhancement of biological reactions on cell surfaces via macromolecular crowding. Nature Communications. 5:4683. doi

David H. Kwan & Stephen G. Withers. Periplasmic de-acylase helps bacteria don their biofilm coat. Proceedings of the National Academy of Sciences. 111(30):10904-5. doi

Ching-Ching Yu, Tara Hill, David H. Kwan, Hong-Ming Chen, Chun-Cheng Lin, Warren Wakarchuk, and Stephen G. Withers. A plate-based high-throughput activity assay for polysialyltransferase from Neisseria meningitidis. Analytical Biochemistry. 444:67-74. doi


Ching-Ching Yu, Li-De Huang, David H. Kwan, Warren W. Wakarchuk, Stephen G. Withers, and Chun-Cheng Lin. A glyco-gold nanoparticle based assay for α-2,8-polysialyltransferase from Neisseria meningitidis. Chemical Communications. 49(86):10166-8. doi


David H. Kwan & Frank Schulz. The stereochemistry of complex polyketide biosynthesis by modular polyketide synthases. Molecules. 16(7):6092-115. doi

David H. Kwan, Manuela Tosin, Nadin Schläger, Frank Schulz, and Peter Leadlay. Insights into the stereospecificity of ketoreduction in a modular polyketide synthase. Organic & Biomolecular Chemistry. 9(7):2053-6. doi

David H. Kwan, Hong-Min Chen, Khakhanang Ratananikom, Susan M. Hancock, Yoichiro Watanabe, Prachumporn T. Kongsaeree, A. Lacey Samuels, and Stephen G. Withers. Self-immobilizing fluorogenic imaging agents of enzyme activity. Angewandte Chemie (International Edition). 50(1):300-3. doi



David H. Kwan & Peter F. Leadlay. Mutagenesis of a modular polyketide synthase enoylreductase domain reveals insights into catalysis and stereospecificity. ACS Chemical Biology. 5(9):829-38. doi



David H. Kwan, Yuhui Sun, Frank Schulz, Hui Hong, Bojana Popovic, Joalice C.C. Sim-Stark, Stephen F. Haydock, and Peter F. Leadlay. Prediction and manipulation of the stereochemistry of enoylreduction in modular polyketide synthases. Chemistry & Biology. 15(11):1231-40. doi

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